Preserved stool samples in DANASTOOL

The DANAGENE MICROBIOME DANASTOOL kit has been designed for a fast and efficient purification of microbial DNA from:

1. Stool homogenate from 0.50-1.0 gr stool and stabilized in 8 ml DANASTOOL .

In this procedure, the microorganisms are efficiently lysed by a combination of heat, chemical and mechanical disruption with specialized beads. Inhibitors are eliminated by precipitation using a proprietary cleanup buffer. The sample is then applied to a microspin column and the DNA that is bound to the column undergoes a single wash step before elution.

Specifications:

• Designed for a fast and easy purification microbial DNA from preserved stool samples in DANASTOOL Sample Collection Microbiome Kit.
• Silica-membrane technology with news microbial DNA columns.
• Optimized lysis method-Combination of heat, chemical and mechanical lysis via bead-based homogenization enables isolation of DNA from yeast, fungi, Gram-negative and Gram-positive bacteria.
• Eliminates inhibitory substances, including lipids, polysaccharides and heme.
• Typical yield: Approx. 5-60 µg depends on sample type.
• Preparation Time: 35 min.
• Elution volume: 200 µl.
• No phenol/cloroform extraction or ethanol precipitation is necessary.

Applications:
• Microbiome analysis
• PCR and qPCR applications.
• Microarrays.
• Mutation analysis