The DANAGENE Microbiome Fecal DNA kit is designed for the efficient isolation of microbial DNA from:
- up to 250 µl fresh and frozen human or animal stool samples.
- 250 µl preserved stool sample stabilized in DANASTOOL Sample Collection MICROBIOME Kit.
- 400 µl preserved stool sample stabilized in DANASWAB Sample Collection MICROBIOME Kit.
Our New DANAGENE Microbiome Fecal DNA kit is even more effective than our original Microbiome Fecal technology and is designed to isolate high yields of pure microbial DNA from stool samples for microbiome and metagenomic analysis.
The kit features a novel Microbial DNA Column and optimized chemistry for a more efficient removal of PCR inhibitors (such as polysaccharides, heme compounds and bile salts) using a novel Microbiome Lysis Buffer and Microbiome Precipitation Buffer
In this procedure, the microorganisms are efficiently lysed by a combination of heat, chemical and mechanical disruption with specialized beads. Proteins and Inhibitors are eliminated by precipitation using a new proprietary inhibitor removal buffer and subsequently pelleted by centrifugation together with the beads and undissolved sample material. The purified lysate is mixed with the Binding Buffer and then applied to a Microbial DNA column. The DNA that is bound to the column undergoes a two wash step. After a drying step, ready to use DNA for NGS, PCR and other downstream applications can be eluted with Elution Buffer (5 mM Tris/HCl, pH 8.5).
Specifications:
• Designed for a fast and easy purification microbial DNA from different types of stool samples.
• Silica-membrane technology with news microbial DNA columns.
• Optimized lysis method-Combination of heat, chemical and mechanical lysis via bead-based homogenization enables isolation of DNA from yeast, fungi, Gram-negative and Gram-positive bacteria.
• Eliminates inhibitory substances, including lipids, polysaccharides and heme.
• Typical yield: Approx. 5-60 µg depends on sample type.
• Preparation Time: 35 min.
• Elution volume: 100-200 µl.
• No phenol/cloroform extraction or ethanol precipitation is necessary.
Applications:
• Microbiome analysis
• PCR and qPCR applications.
• Microarrays.
• Mutation analysis
