The new DANAGENE MICROBIOME Soil DNA kit has been designed for the isolation of high molecular weight genomic DNA from microorganisms like Gram positive and Gram negative bacteria, archaea and fungi from environmental samples of all soil types.
The kit uses a new Microbial DNA Columns, Microbiome Lysis Buffer and Microbiome Precipitation Buffer specifically designed for use with environmental samples containing high humic substances content, including difficult soil types.
In this procedure, the microorganisms are efficiently lysed by a combination of heat, chemical and mechanical disruption with specialized beads. Proteins and Inhibitors are eliminated by precipitation using a new proprietary inhibitor removal buffer and subsequently pelleted by centrifugation together with the beads and undissolved sample material. The purified lysate is mixed with the Binding Buffer and then applied to a Microbial DNA column. The DNA that is bound to the column undergoes a two wash step. After a drying step, ready to use DNA can be eluted with Elution Buffer (5 mM Tris/HCl, pH 8.5).
Specifications:
• Designed for a fast and easy purification microbial DNA from different types of soil samples.
• Optimized lysis method-combination of heat, chemical and mechanical lysis via bead-based homogenization enables isolation of DNA from archaea, fungi, Gram-negative and Gram-positive bacteria.
• Eliminates inhibitory substances as humic substances and others inhibitors.
• No phenol/chloroform extraction or ethanol precipitation is necessary.
• Typical yield: Approx. 1-20 µg depends on sample type.
• Preparation Time: 35 min.
• Elution volume: 50-100 µl.
• The eluted DNA is ready to use for all standard downstream applications. In most cases the concentrated DNA can be used as PCR template without further dilution for highest sensitivity.
Applications:
• Microbiome analysis.
• PCR and Real-time applications.
• Microarrays.
• Mutation analysis.
