Title Image

Saliva

Home  /  DNA Purification Kits  /  Saliva

We presented a different kits for an efficient and fast  purification of highly pure genomic DNA from a wide variety of saliva samples including:

 

DANAGENE SALIVA DNA Kit

1. Saliva samples.
2. Preserved Saliva samples with our DANASALIVA  Sample Collection Kit. Ref.0603.43
3. Preserved Saliva samples with the ORAGENE self collection kits (DNAGenotek).

 

DANAGENE SWABS DNA Kit

1. Danagene’s buccal swabs.
2. Preserved buccal swabs with our DANASWABS Sample Collection Kit. Ref.0615

The process includes a cell lysis with an anionic detergent that solubilizes the necessary cell components, proteinase K and RNase. The cell proteins are removed by precipitation, which allows to leave the genomic DNA in solution. Finally, the genomic DNA is isolated by a precipitation with isopropanol.

Specifications:
• DNA from saliva is equivalent to DNA from blood for downstream applications.
• Improve patient care and compliance with painless, non-invasive sample collection and decreases costs.
• Reproducible, fast and non-expensive method.
• Safe method, as it removes completely the need of using toxic reagents.

 

Applications: DNA purified using this kit is highly stable and suited for use in a wide range of applications such as:

• DNA archiving.
• PCR and quantitative real-time PCR.
• SNP analysis.
• Southern Blotting.
• Next Generation Sequencing.

Reference Product Description Preps
0603.4 DANAGENE SALIVA DNA kit 50
0603.41 DANAGENE SALIVA DNA kit 160
Reference Product Description Preps
0616.100 DANAGENE SWABS DNA kit 100
0616.500 DANAGENE SWABS DNA kit 500
0616.1000 DANAGENE SWABS DNA kit 1000

DANAGENE SPIN SALIVA DNA kit is designed for the rapid purification of highly pure genomic DNA from saliva samples using silica-based system with a MicroSpin format:

 

a) Preserved saliva samples in the DANASALIVA Sample Collection Kit. 

b) Fresh saliva samples.

Procedure: The process includes a cell lysis by incubation of the sample in a lysis buffer and proteinase K at 55°C. Appropriate conditions for binding of DNA to the glass fiber membrane are created by addition of large amounts of chaotropic ions to the lysate. Contaminants are removed by efficient washing with two wash buffer. Pure genomic DNA is finally eluted with an elution buffer and it’s ready to use for subsequent reactions.

Specifications

 

• Silica membrane technology with Mini spin columns.
• Sample Size: 400 ml preserved saliva sample in DANASALIVA Sample Collection Kit; 600-800 ml of fresh saliva.
• Complete removal of contaminants and inhibitors for reliable downstream applications.
• A260/280 > 1.75; A230/260 > 2.00
• Elution volume 50-100 m
• High quality DNA obtained that can be directly used in PCR, Southern, NGS, etc.

Reference Product Description Preps
0603.SPIN50 DANAGENE SPIN Saliva DNA kit 50
0603.SPIN250 DANAGENE SPIN saliva DNA kit 250