This kit has been optimized for an efficient and fast purification of total DNA from :
- Fresh/frozen stool samples.
- Various food samples (raw material and processed food).
After the samples have been homogenized, the DNA can be extracted with the extraction buffer, lysis mixtures should be cleared by centrifugation or filtration in order to remove contaminants and residual cellular debris. The clear supernatant is then mixed with the binding buffer, proteinase K and isopropanol to create conditions for optimal binding to the silica membrane column. After washing with two different buffers for efficient removal of potential PCR inhibitors, DNA can be eluted in low salt buffer or water, and is ready-to-use in subsequent reactions.
Specifications:
• Silica Membrane Technology using MiniSpin columns.
• Sample size: up to 200mg.
• Complete removal of contaminants and inhibitors for reliable downstream applications.
• Simultaneous extraction of microbial DNA and host DNA.
Applications:
• DNA extracted from stool samples is an important tool in different areas of molecular genetic research reaching from cancer diagnostics to population genetic studies.
• DNA from complex matrices, processed food, soya, chocolat, cereals, meat, animal feed.
• Detection of genetically modified material in food products.
• Detection of specific DNA in animal feed.
| Reference | Product Description | Preps |
| 0609.1 | DANAGENE SPIN FOOD-STOOL kit | 50 |
| 0609.2 | DANAGENE SPIN FOOD-STOOL kit | 250 |
This kit has been optimized for an efficient and fast PCR-ready bacterial DNA extraction (Listeria, Salmonella, E.coli, etc) from pre-enrichment or enrichment culture from different food samples, raw materials or feces using glass fiber membrane MicroSpin columns which selectively bounds the DNA.
Detection of very low levels of bacterial contamination in foods and feces necessitates these samples to be cultured for a few hours in an appropriate enrichment broth. The use of culture enrichment prior to PCR analysis serves many purposes, including:
1) Dilution of PCR inhibitory substances present in the sample matrix.
2) Multiplication of the target organism to provide detectable concentrations.
3) Dilution of dead cells.
4) Possibility of isolating the target organism for complementary tests.
The procedure includes a centrifugation of 1 ml of enrichment medium to concentrate the cells, the cells are lysed during an incubation period with Lysis Solution and Lysozyme (supplied with the kit). Then a digestion with Proteinase K (supplied with the kit) and cleaning of the lysis mixture by centrifugation and DNA binding to the glass fiber membranes packed in MicroSpin columns. The DNA that is bound is purified by several washes to eliminate the inhibitory potentials of the PCR and finally the DNA is eluted.
Specifications:
• Silica Membrane Technology using MiniSpin columns.
• Complete removal of PCR inhibitors.
• PCR and Real Time PCR- ready DNA.
• Sample size: from 1 ml of pre-enrichment or enrichment medium of different food samples.
| Reference | Product Description | Preps |
| 0608.1 | DANAGENE SPIN FOOD-STOOL “Bacteria” kit | 50 |
| 0608.2 | DANAGENE SPIN FOOD-STOOL “Bacteria” kit | 250 |