The DANAGENE MICROBIOME Swab DNA kit has been designed for a fast and efficient purification of microbial DNA from preserved samples using our DANASWAB Sample Collection MICROBIOME Kit for microbiome analysis.
In this procedure, the microorganisms are efficiently lysed by a combination of heat, chemical and mechanical disruption with specialized beads. Inhibitors are eliminated by precipitation using a proprietary cleanup buffer. The sample is then applied to a microspin column and the DNA that is bound to the column undergoes a single wash step before elution.
High-Quality DNA
DNA was isolated from aliquots of 800μl of preserved feces samples with our DANASWAB Sample Collection MICROBIOME kit. These samples were preserved at room temperature for 30 days. Isolation was carried out on day 1, day 15 and day 30 using our DANAGENE MICROBIOME Swab DNA Kit. Quality was assessed using the Agilent 4150 TapeStation.
Features:
• Designed for a fast and easy purification microbial DNA from preserved samples using our DANASWAB Sample Collection MICROBIOME Kit.
• Optimized lysis method-combination of heat, chemical and mechanical lysis via bead-based homogenization enables isolation of microbial DNA for microbiome analysis.
• Eliminates inhibitory substances.
• No phenol/chloroform extraction or ethanol precipitation is necessary.
Aplications:
• Microbiome analysis
• PCR applications.
• RFLP analysis.
• Patogen typing.
